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Co-transfection of wild-type (WT) Ran with PlexinB1 also results in Cos7 cell collapse to a similar extent to transfection of PlexinB1 alone

PROTEINDEX HiBond Ni-NTA Agarose utilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni2+) to form a very stable octahedral structure with the nickel ion in the center

doi: 10.1210/jcem.85.8.6720 74 BastardJPJardelCBruckertEBlondyPCapeauJLavilleMet al

This result does not support a Golgi-to-PM flow of MGNC-Nt 7 -FM 4 -GFP, as PM targeting was not preceded by its Golgi accumulation